This Article Full Text (PDF) All Versions of this Article: 0885328207083311v1 0885328207083311v2 22/6/559    most recent References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Maus, U. Articles by Siebert, C. H. Search for Related Content PubMed PubMed Citation Articles by Maus, U. Articles by Siebert, C. H. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB TRICALCIUM PHOSPHATE Social Bookmarking                         What's this?

BMP-2 Incorporated in a Tricalcium Phosphate Bone Substitute Enhances Bone Remodeling in Sheep

Department of Orthopedic Surgery, University of Aachen, Pauwelsstr 30, D-52074 Aachen, Germany, umaus{at}ukaachen.de

Stefan Andereya

Department of Orthopedic Surgery, University of Aachen, Pauwelsstr 30, D-52074 Aachen, Germany

Sascha Gravius

Department of Orthopedic Surgery, University of Aachen, Pauwelsstr 30, D-52074 Aachen, Germany

Jörg A.K. Ohnsorge

Department of Orthopedic Surgery, University of Aachen, Pauwelsstr 30, D-52074 Aachen, Germany

Christopher Niedhart

Department of Orthopedic Surgery, University of Aachen, Pauwelsstr 30, D-52074 Aachen, Germany

Christian H. Siebert

Orthopaedic Clinic I, Annastift e.V. Anna-von-Borries-Str. 17 D-30625 Hanover, Germany

Bone morphogenetic protein-2 (BMP-2) is a well-known osteoinductive protein, which requires a carrier for local application. As an alternative to the previously described carriers, an in situ hardening, resorbable, and osteoconductive β-tricalcium phosphate cement (TCP) is tested. Trepanation defects in the bovine distal femoral epiphysis are filled with a composite consisting of TCP and 200 µg rhBMP-2 per cm³ TCP, autologous bone graft, pure TCP, or left empty. A radiological follow-up is performed after 7 weeks and 3 months. The sheep are euthanized and bone samples are analyzed by microradiography, histology, and histomorphometry. Microradiography and histology show similar results for pure TCP and the composite. The defects are filled with trabecular bone and newly formed bone is in close contact with the remaining TCP-particles. The majority of the cement is resorbed, in the composite group the amount of remaining cement particles is reduced. Defects treated with autologous bone graft are filled completely, while untreated defects shows only a small amount of bone originating from the rim of the defect. Histomorphometry of the defects treated with pure TCP shows a significantly increased bone content in comparison to defects treated with the composite or autologous bone graft. Analysis of the remaining cement particles shows significantly less cement in the TCP/rhBMP-2 group in comparison to pure TCP. The sum of bone and cement content in the rhBMP-2 group shows amounts comparable to the calcified structures found following autologous bone grafting. The addition of rhBMP-2 to the TCP leads to faster remodeling of the defect comparable to autologous bone graft, while defects treated with pure TCP are not completely remodeled.

Key Words: BMP-2 • bone substitute • TCP • calcium phosphate • osteogenesis • osteoinduction • osseous defect.

This version was published on May 1, 2008

Journal of Biomaterials Applications, Vol. 22, No. 6, 559-576 (2008)
DOI: 10.1177/0885328207083311


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?