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Journal of Biomaterials Applications
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Article

Compatibility of Embryonic Stem Cells with Biomaterials

Jorg Handschel1, Karin Berr1, Rita Depprich1*, Christian Naujoks1, Norbert R. Kubler1, Ulrich Meyer1, Michelle Ommerborn2, and Lydia Lammers3

1 Department for Cranio- and Maxillofacial Surgery Heinrich-Heine-Universität, Moorenstr. 5, D-40225 Düsseldorf, Germany
2 Department for Operative and Preventive Dentistry and Endodontics, Heinrich-Heine-University Düsseldorf Moorenstr. 5, D-40225 Düsseldorf, Germany
3 Department for Cranio- and Maxillofacial Surgery Westfälische-Wilhelms-Universität, Waldeyerstr. 30 D-48149 Münster, Germany

* To whom correspondence should be addressed.


  Abstract

Periodontal bone defects and atrophy of the jaws in an aging population are of special concern. Tissue engineering using embryonic stem cells (ESCs) and biomaterials may offer new therapeutic options. The purpose of this study is to evaluate the compatibility of ESCs with biomaterials and the influence of biomaterials on the osteogenic gene expression profile.

Therefore, ESCs are cultured with various biomaterials. The cytocompatibility of murine ESCs is measured regarding the proliferation of the cells on the materials by CyQUANT® assay, the morphology by scanning electron microscopy, and the influence on the gene expression by real time PCR.

The results show that insoluble collagenous bone matrix, followed by {beta}-tricalciumphosphate, is most suitable for bone tissue engineering regarding cell proliferation, and phenotype. The gene expression analysis indicates that biomaterials do influence the gene expression of ESCs.

Our results provide new insight into the cytocompatibility of ESCs on different scaffolds.

Key Words: bone tissue engineering, biomaterials, embryonic stem cells, compatibility.

First published on August 29, 2008, doi:10.1177/0885328208094305

Journal of Biomaterials Applications 2009;23:549.

A more recent version of this article appeared on May 1, 2009

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