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Journal of Biomaterials Applications
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Article

Seeding Osteoblastic Cells into a Macroporous Biodegradable CaP/PLGA Scaffold by a Centrifugal Force

Marcio Mateus Beloti1, Paulo Tambasco De Oliveira1, Adalberto Luiz Rosa1*, and Paulo Sergio Perri De Carvalho2

1 Cell Culture Laboratory, School of Dentistry of Ribeirao Preto University of Sao Paulo, Ribeirao Preto, 14040-904, SP, Brazil
2 Department of Stomatology, School of Dentistry of Bauru University of Sao Paulo, Bauru, 17012-90,1 SP, Brazil

* To whom correspondence should be addressed.


  Abstract

This study aims to construct a hybrid biomaterial by seeding osteoblastic cells into a CaP/PLGA scaffold by a centrifugal force. Constructs are evaluated with respect to potential application in bone tissue engineering. Cells adher, spread, and form a layer of tissue lining the scaffold and are capable of migrating, proliferating, and producing mineralized matrix. We have demonstrated that the centrifugal force is highly efficient for constructing a hybrid biomaterial, which acts similarly to bone explants in a cell culture environment. In this way, these constructs could mimic an autogenous bone graft in clinical circumstances. Such a strategy may be useful for bone tissue engineering.

Key Words: scaffold, cell seeding, centrifugal force, osteoblast, bone tissue engineering.

First published on September 18, 2008, doi:10.1177/0885328208094082

Journal of Biomaterials Applications 2009;23:481.

A more recent version of this article appeared on May 1, 2009

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