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Journal of Biomaterials Applications
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Article

Three-Dimensional Cell Growth on Structures Fabricated from ORMOCER® by Two-Photon Polymerization Technique

Sabrina Schlie1, Anaclet Ngezahayo1*, Aleksandr Ovsianikov2, Tilman Fabian3, Hans-Albert Kolb1, Heinz Haferkamp3, Boris N. Chichkov2

1 Institute of Biophysics, University Hannover, Herrenhäuserstr. 2, D-30419 Hannover, Germany
2 Laser Zentrum Hannover e.V., Hollerithallee 8, D-30419 Hannover, Germany
3 Zentrum für Biomedizintechnik, Universität Hannover, Lise-Meitner-Str. 1 D-30823 Garbsen, Germany

* To whom correspondence should be addressed.


  Abstract

Two-photon polymerization technique was applied to generate three-dimensional (3D) scaffold-like structures using the photosensitive organic-inorganic hybrid polymer ORMOCER®. The structures were studied with respect to potential applications as scaffold for tissue engineering. Cell counting and comet assay, respectively, demonstrated that doubling time and DNA strand breaks of CHO cells, GFSHR-17 granulosa cells, GM-7373 endothelial cells, and SH-SY5Y neuroblastoma cells were not affected by ORMOCER®. ORMOCER® related alteration of formation of tissue specific cell-to-cell adhesions like gap junctions was ruled out by double whole-cell patch-clamp technique. Additionally, growth of cells on the vertical surfaces of 3D structures composed of ORMOCER® is shown.

Key Words: tissue engineering, two-photon polymerization, ORMOCER®, 3D scaffolds, gap junction, comet assay, DNA strand breaks

First published on May 10, 2007, doi:10.1177/0885328207077590

Journal of Biomaterials Applications 2007;22:275.

A more recent version of this article appeared on November 1, 2007

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