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Journal of Biomaterials Applications
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*HYDROXYAPATITE
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Article

Three-dimensional Nanohydroxyapatite/Chitosan Scaffolds as Potential Tissue Engineered Periodontal Tissue

Yu-Feng Zhang1, Xian-Rong Cheng1*, Yun Chen2, Bin Shi1, Xiao-Hui Chen1, Dong-Xuan Xu1, Jin Ke1

1 Ministry Education Key Laboratory for Oral Biomedical Engineering School of Stomatology, Wuhan University, Wuhan 430079, P. R. China
2 Hubei Key Laboratory for Allergy and Immune-related Diseases and Center for Medical Research, School of Medicine Wuhan University, Wuhan 430072, P. R. China

* To whom correspondence should be addressed.


  Abstract

The development of suitable three-dimensional scaffold for the maintenance of cellular viability and differentiation is critical for applications in periodontal tissue engineering. In this work, different ratios of porous nanohydroxyapatite/chitosan (HA/chitosan) scaffolds are prepared through a freeze-drying process. These scaffolds are evaluated in vitro by the analysis of microscopic structure, porosity, and cytocompatibility. The expression of type I collagen and alkaline phosphatase (ALP) activity are detected with real-time polymerase chain reaction (RT-PCR). Human periodontal ligament cells (HPLCs) transfected with enhanced green fluorescence protein (EGFP) are seeded onto the scaffolds, and then these scaffolds are implanted subcutaneously into athymic mice. The results indicated that the porosity and pore diameter of the HA/chitosan scaffolds are lower than those of pure chitosan scaffold. The HA/chitosan scaffold containing 1% HA exhibited better cytocompatibility than the pure chitosan scaffold. The expression of type I collagen and ALP are up-regulated in 1% HA/chitosan scaffold. After implanted in vivo, EGFP-transfected HPLCs not only proliferate but also recruit surrounding tissue to grow in the scaffold. The degradation of the scaffold significantly decreased in the presence of HA. This study demonstrated the potential of HA/chitosan scaffold as a good substrate candidate in periodontal tissue engineering.

Key Words: tissue engineering, chitosan, hydroxyapatite, scaffold, periodontal ligament, cell

First published on March 16, 2006, doi:10.1177/0885328206063853

Journal of Biomaterials Applications 2007;21:333.

A more recent version of this article appeared on April 1, 2007

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