Dynamics of Subcutaneous Tissue Response to the Implantation of
Tetracycline-Treated or Untreated Membrane of Demineralized Bovine Cortical Bone in Rats
Rodrigo Cardoso De Oliveira1,
Everdan Carneiro1,
Tania Mary Cestari1,
Rumio Taga1,
Jose Mauro Granjeiro2*
1 Department of Biological Sciences, Bauru Dental School, University of
São Paulo, Brazil
2 epartment of Biological Sciences, Bauru Dental School, University of
São Paulo, Brazil
* To whom correspondence should be addressed.
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Abstract |
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This study aims to conduct a histological evaluation of tissue response to a
membrane obtained from demineralized bovine cortical bone, associated or not, to
tetracycline (TTC). TTC treated and untreated bovine membranes were implanted in the
subcutaneous tissue of rats (n = 120). The animals were killed 1, 3, 7, 15, 30,
and 60 days after surgery. The tissue around the material was fixed in 10% buffered
formalin for 24 h. Sections of 6 µm were stained with hematoxylin and eosin. In
general, moderate to intense inflammatory response was observed in the initial periods
(1 and 3 days), moderate response in the 7- and 15-day periods, and that was remarkably
reduced at 30 and 60 days. Resorptions of the membranes by mononuclear cells
(fibroblasts and macrophages) and multinucleated giant cells were observed 15 days after
implantation. Only the remnants of the material could be detected in some animals in 60
days. Both membranes were tolerated by the tissue and were completely resorbed after
30-60 days. While the association of TTC apparently accelerated the biodegradability of
the membrane substrate, no significant differences were found in the tissue response
behavior between the two groups tested.
Key Words:
membranes, tetracycline, biological barriers, bovine bone
