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Journal of Biomaterials Applications
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Preparation of Graded Zirconia—Hydroxyapatite Composite Bioceramic and Its Immunocompatibility in vitro

Renfu Quan

Department of Orthopaedics, Second Affiliated Hospital Zhejiang University, 310009 Hangzhou, China

Disheng Yang

Department of Orthopaedics, Second Affiliated Hospital Zhejiang University, 310009 Hangzhou, China, dishengyangzj{at}yahoo.com

Xudong Miao

Department of Orthopaedics, Second Affiliated Hospital Zhejiang University, 310009 Hangzhou, China

Xiaochun Wu

School of Materials Science and Engineering Shanghai University, 200072 Shanghai, China

Hongbin Wang

School of Materials Science and Engineering Shanghai University, 200072 Shanghai, China

Wei Li

Department of Orthopaedics, Xiaoshan Traditional Chinese Medical Hospital, 311201 Hangzhou, China

To obtain immunocompatibility, graded zirconia—hydroxyapatite (ZrO 2-HA) composite bioceramic and simplex ZrO2-HA composite bioceramic are prepared. Peripheral blood mononuclear cells (PBMCs) harvested from healthy individuals are cultured with the two ceramic extracts to assess their effect on the transformation of lymphocytes, apoptotic ratio, CD69 expression, and expression of cytokine of TNF{alpha} and IL-6, with or without phytohemoagglutinin (PHA)-stimulated cells. Ceramic extracts did not activate the resting lymphocytes, whereas the response of the PHA-stimulated cells was significantly modified. The PBMCs activated by graded ZrO2-HA composite bioceramic is noticeably smaller than that by simplex ZrO2-HA composite bioceramic; these results, however can only be seen under the amplified effect of PHA-stimulation.

Key Words: zirconia • hydroxyapatite • graded composite material • biocompatibility • immunocompatibility.

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This version was published on September 1, 2007

Journal of Biomaterials Applications, Vol. 22, No. 2, 123-144 (2007)
DOI: 10.1177/0885328206071454
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*HYDROXYAPATITE
*ZIRCONIUM
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