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Effect of Ganciclovir on the Hydrolytic Degradation of Poly(lactide-co-glycolide) Microspheres

Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, 639798 Singapore

C. P. Ooi

Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, 639798 Singapore, ascpooi{at}ntu.edu.sg

T. H. Lim

Department of Ophthalmology, Tan Tock Seng Hospital, 308433 Singapore

Ganciclovir (GCV)-loaded poly(lactide-co-glycolide) (PLGA) microspheres, 125 ± 11 µm in diameter, are produced using the emulsification/solvent evaporation technique. The release rate of the drug is studied for 20 weeks in a phosphate-buffered solution of pH 7 at 37°C. The release of the drug shows a triphasic release pattern, i.e., an initial burst, a diffusive phase, and a second burst. The initial burst occurs within the first 2 days of immersion. After the burst, the release is by diffusion for up to 13 weeks, followed by another burst release, which signals the onset of bulk degradation of the PLGA polymer. The presence of GCV molecules decreases the hydrolytic rate of PLGA degradation. Gel permeation chromatography (GPC), differential scanning calorimetry (DSC), field emission scanning electron microscopy (FESEM), and ultraviolet (UV) spectroscopy are used to assess the hydrolytic degradation and drug release rate of the microspheres.

Key Words: poly(lactide-co-glycolide) • ganciclovir • microspheres • triphasic release • hydrolytic degradation

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Journal of Biomaterials Applications, Vol. 20, No. 3, 287-302 (2006)
DOI: 10.1177/0885328206054265


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This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Web of Science (4) Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Chen, X. Articles by Lim, T. H. Search for Related Content PubMed PubMed Citation Articles by Chen, X. Articles by Lim, T. H. Social Bookmarking                         What's this?