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Modifications on Collagen Structures Promoted by 1,4-Dioxane Improve Thermal and Biological Properties of Bovine Pericardium as a Biomaterial

Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, CEP 05508-900, São Paulo-SP, Brasil, flforti{at}quim.iq.usp.br

Gilberto Goissis

Departamento de Química e Física Molecular, Instituto de Química de São Carlos, Universidade de São Paulo, CEP 13560-970, São Paulo-SP, Brasil

Ana M. G. Plepis

Departamento de Química e Física Molecular, Instituto de Química de São Carlos, Universidade de São Paulo, CEP 13560-970, São Paulo-SP, Brasil

Collagen is a widely used raw material for biomaterial manufacture, which generally depends on chemical modifications of this fibrillar protein with cross-linking agents to improve biocompatibility and mechanical properties. However, cross-linking reduces the natural properties of collagen, such as low immune response, low toxicity as well as the ability to promote cellular growth and attachment. In this work, the modifications promoted by 1,4-dioxane solvent on the collagen present in native bovine pericardium (NBP) matrix routinely used in bioprosthesis manufacture, with or without subsequent cross-linking by glutaraldehyde, has been studied. The structural changes of NBP evaluated by scanning electron microscopy show that 1,4-dioxane induces a more homogeneous material by increasing aggregation of collagen fibers, while transmission scanning electron microscopy shows that natural collagen fibril arrangement, integrity, and the D-periodicity pattern are maintained by solvent treatments. Measurements of thermal stability and resistance to collagenase enzymatic digestion of NBP matrices treated with 1,4-dioxane show an increase in melting temperature and decrease in biodegradability, as compared to native pericardium. Cross-linking with glutaraldehyde improves all the analyzed NBP properties, which are not impaired by previous treatment with 1,4-dioxane. Histological evaluation of NBP submitted to 1,4-dioxane treatment shows lower lipid and cell contents and improvement in other morphologic characteristics compared to native pericardium. Altogether, these results suggest the use of 1,4-dioxane organic solvent as an alternative non-cross-linking treatment for direct utilization on rich collagen matrices, resulting in materials with improved biocompatibility and physicochemical properties suitable for tissue engineering.

Key Words: 1,4-dioxane • collagen • bovine pericardium • biomaterials • glutaraldehyde

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Journal of Biomaterials Applications, Vol. 20, No. 3, 267-285 (2006)
DOI: 10.1177/0885328206054048


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This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Web of Science (1) Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Forti, F. L. Articles by Plepis, A. M. G. Search for Related Content PubMed PubMed Citation Articles by Forti, F. L. Articles by Plepis, A. M. G. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB 1,4-DIOXANE Social Bookmarking                         What's this?