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Journal of Biomaterials Applications
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Chemical-Physical Characterization and in vitro Preliminary Biological Assessment of Hyaluronic Acid Benzyl Ester-Hydroxyapatite Composite

C. Giordano

Department of Chemistry, Materials and Chemical Engineering ‘Giulio Natta’, Polytechnic of Milan, Via L. Mancinelli 7-20131 Milan, Italy, carmen.giordano{at}polimi.it

V. Sanginario

Institute of Composite and Biomedical Materials, IMCB-CNR, Piazzale Tecchio 80, 80125 Naples, Italy

L. Ambrosio

Institute of Composite and Biomedical Materials, IMCB-CNR, Piazzale Tecchio 80, 80125 Naples, Italy

L. Di Silvio

Guy’s, King’s and St Thomas’ Medical and Dental Institute, King’s College London, Biomaterials Science, Guy’s Hospital, St Thomas’ Street, London SE1 9RT, UK

M. Santin

School of Pharmacy & Biomolecular Sciences, University of Brighton, Cockcroft Building, Lewes Road, Brighton BN2 4GJ, UK

HYAFF®11 is a biocompatible, biodegradable benzyl ester of hyaluronic acid. However, in order to use it for orthopedic application, its mechanical performance needs to be improved. In this study, a novel composite based on HYAFF®11 polymer matrix reinforced with hydroxylapatite (HA) has been developed. Its advantage is having a similar component of the mineral phase of bone resulting in favorable osteoconductive properties. The present study has examined the compressive mechanical and surface chemical-physical properties of the novel HYAFF®11-HA composite. Preliminary biological investigations, including pH and cytotoxicity studies of the material extracts, have also been performed using an in vitro primary human osteoblast-like cell model. Moreover, protein, especially fibronectin adsorption has been investigated following incubation in culture medium and human plasma. The results show a grainy surface topography composed mainly of C, P, and Ca, with a Ca/P atomic ratio indicating HA on the composite surface. Mechanical analysis shows an improvement of the compressive properties of HYAFF11 matrix, both in the dry and swollen states, with values in the range of that of spongy bone. No cytotoxic effects and no inhibition of cell proliferation have been observed in the presence of the material extracts with pH values within acceptable ranges for cell vitality. Protein studies reveal a similar pattern, but a higher amount of fibronectin following incubation in human plasma when compared with culture medium. The results show that the novel HYAFF®11-HA composite shows a great potential for application in orthopedic fields, especially as vertebral trabecular bone substitute.

Key Words: hydrogel • composite • surface analysis • stress analysis • cell response • protein adsorption • biocompatibility • osteoblast • in vitro tests • cell culture

References

  • Laurent, T.C. and Fraser, J.R. (1992). Hyaluronan , FASEB J., 6(7): 2397-2404 .[Abstract]
  • Campoccia, D., Doherty, P., Radice, M., Brun, P., Abatangelo, G. and Williams, D.F. (1998). Semisynthetic Resorbable Materials Form Hyaluronan Esterification , Biomaterials, 19: 2101-2127 .[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Iannace, S., Ambrosio, L., Nicolais, L., Rastrelli, A. and Pastorello, A. (1992). Thermomechanical Properties of Hyaluronic Acid Derived Products , J. Mater. Sci. Mater. Med., 3(1): 59-64 .
  • LeGeros, R.Z. and LeGeros, J.P. (1993). Dense Hydroxyapatite, In: Hench, L.L. and Wilson, J. (eds), An Introduction to Bioceramics, Chap 9, pp. 139-180, World Scientific, Singapore .
  • Ozawa, S. and Kagusai, S. (1996). Evaluation of Implant Materials (Hydroxyapatite, Glass Ceramics, Titanium) in Rat Bone Marrow Stromal Cell Culture , Biomaterials, 17: 23-29 .[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • De Groot, K., Wolke, J.G.C. and Jansen, J. (1998). Calcium Phosphate Coatings for Medical Implants, Proceeding of the Institution of Mechanical Engineering , Journal of Engineering in Medicine, 212-H: 137-147 .
  • Di Silvio, L. and Gurav, N. (2001). Osteoblasts, In: Koller, M.R., Palsonn, B.O. and Masters, J.R.W. (eds), Human Cell Culture, Vol. V, Chap. 11, pp. 221-241, Kluwer Academic Publisher, UK .
  • Laemmli, U.K. (1970). Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , Nature, 277: 680-685 .
  • Netti, P.A., Ambrosio, L. and Nicolais, L. (2000). Thermodynamics of Water Sorption in Hyaluronic Acid and its Derivative, In: Ottenbrite, R.M. and Kim, S.W. (eds), Polymers for Medical Applications in the Twenty First Century, Chap. 12, pp. 169-180, Tehnomics Publishing Co., Lancaster, PA, USA .
  • Morgan, E.F. and Keaveny, T.M. (2001). Dependence of Yield Strain of Human Trabecular Bone on Anatomic Site , J. of Biomechanics, 34: 569-577 .
  • Curtis, A. and Wilkinson, C. (1999). New Depths in Cell Behaviour: Reactions of Cells to Nanotopography , Biochem. Soc. Symp., 65: 15-26 .[Medline] [Order article via Infotrieve]
  • Curtis, A.S. and Wilkinson, C.D. (1998). Reaction of Cells to Topography , J. Biomater Sci. Polym. Ed., 9: 1313-1329 .[Medline] [Order article via Infotrieve]
  • Harris, S.A., Enger, R.J., Riggs, B.L. and Spelsberg, T.C. (1995). Development and Characterisation of a Conditionally Immortalised Human Fetal Osteoblastic Cell Line , J. Bone Miner. Res., 10: 178-186 .[Web of Science][Medline] [Order article via Infotrieve]
  • Larsson, C., Thomsen, P., Lausmaa, J., Rodhal, M., Kasemo, B. and Ericon, LE. (1994). Bone Response to Surface Modified Titanium Implants with Different Oxide Thickness and Morphology , Biopmater, 15: 1061-1074 .
  • Kasemo, B. and Lausmaa, J. (1994). Material-Tissue Interfaces: the Role of Surface Properties and Processes , Environ. Health Prospects, 102: 241-245 .
  • Kasemo, B. and Lausmaa, J. (1986). Surface Science Aspects of Inorganic Biomaterials , Crit. Rev. Biocompat., 2: 335-380 .
  • Stephenson, P.K., Freeman, M.A., Revell, P.A., Germain, J., Tuke, M. and Pirie, C.J. (1991). The Effect of Hydroxyapatite Coating on Ingrowth of Bone into Cavities of an Implant , J. Arthoplasty., 6: 51-58 .
  • Ohgushi, H., Dohi, Y., Yoshikawa, T., Tamai, S., Tabata, S., Okunaga, K. and Shibuya, T. (1996). Osteogenic Differentiation of Cultured Marrow Stromal Stem Cells on the Surface of Bioactive Glass Ceramics , J. Biomed. Mat. Res., 32: 341-348 .[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Ohgushi, H., Dohi, Y., Yoshikawa, T., Tamai, S., Tabata, S. and Suwa, Y. (1996). In vitro Bone Formation by Rat Marrow Cell Culture , J. Biomed. Mat. Res., 32: 333-340 .[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Posner, A.S. and Betts, F. (1975). Synthetic Amorphous Calcium Phosphate and its Relation to Bone Mineral Structure , Acc. Chem. Res., 58: 273-281 .[CrossRef]
  • Dalby, M.J., Kayser, M.V., Bonfield, W. and Di Silvio, L. (2002). Initial Attachment of Osteoblasts to an Optimised HAPEX Topography , Biomaterials, 23: 681-690 .[Medline] [Order article via Infotrieve]
  • Davis, P.A., Huang, S.J., Nicolais, L. and Ambrosio, L. (1991). In: Szycher, M. (ed.), High Performance Biomaterials, pp. 343-368, Technomic Publishing, Lancaster, PA, USA .
  • El-Ghannam, A., Duchyne, P. and Shapiro, I. (1993). A New Bioactive Glass Template for the Synthesis Bone-like Tissue in vitro, In: Ducheyne, P. and Christiansen, D. (eds). Bioceramics 6, pp. 143-149, Butter-Worth-Heinemann, Oxford .

Journal of Biomaterials Applications, Vol. 20, No. 3, 237-252 (2006)
DOI: 10.1177/0885328206051811
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This Article
Right arrow Abstract Freely available
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Right arrow Articles by Giordano, C.
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Right arrow Articles by Santin, M.
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What's this?