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Journal of Biomaterials Applications
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Evaluation of Parenteral Depot Insulin Formulation using PLGA and PLA Microparticles

Pratap C. Naha

National Institute of Immunology, Aruna Asaf Ali Marg New Delhi 110067, India

Vibhu Kanchan

National Institute of Immunology, Aruna Asaf Ali Marg New Delhi 110067, India

Amulya K. Panda

National Institute of Immunology, Aruna Asaf Ali Marg New Delhi 110067, India, amulya{at}nii.res.in

PLGA and PLA microparticles entrapping insulin are prepared by solvent evaporation method and are evaluated in diabetes-induced rat for its efficacy in maintaining blood sugar level from a single intramuscular dose. In vitro release of insulin from PLGA and PLA microparticles are 75.35 ± 1.73% and 67.536 ± 2.23%, respectively in 168 h (7 days). Released insulin from polymer particles are mostly in monomeric form without aggregation. Optimal use of stabilizers during particle formulation helps in reducing protein denaturation and thus results in stabilized insulin-loaded polymer particles. Intramuscular administration of insulin-loaded PLGA (50 : 50) and PLA microparticles (equivalent to 25 IU insulin/kg of animal weight) in alloxaninduced diabetic rats result in 53.86 ± 4.2% and 39.52 ± 6.7% reduction in blood glucose level, respectively in 96 h. This effect continued up to 7 days in case of PLGA and PLA microparticles.

Key Words: insulin • intramuscular delivery • PLGA • PLA • microparticles • hypoglycemic.

This version was published on November 1, 2009

Journal of Biomaterials Applications, Vol. 24, No. 4, 309-325 (2009)
DOI: 10.1177/0885328208096238
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