Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

CiteULike is a free service for managing and discovering scholarly references - click here to get started.

Sign In to gain access to subscriptions and/or personal tools.
Journal of Biomaterials Applications
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
0885328208094301v1
23/6/533    most recent
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Zigang Ge
Right arrow Articles by Tan, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zigang Ge,
Right arrow Articles by Tan, E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Proliferation and Differentiation of Human Osteoblasts within 3D printed Poly-Lactic-co-Glycolic Acid Scaffolds

Zigang Ge

Peking University, Biomedical Engineering, National University of Singapore, Oral and Maxillofacial Surgery, Singapore

Lishan Wang

Institute of Bioengineering and Nanotechnology, Singapore

Boon Chin Heng

National University of Singapore, Oral and Maxillofacial Surgery, Singapore

Xian-Feng Tian

National University of Singapore, Oral and Maxillofacial Surgery, Singapore

Kai Lu

National University of Singapore, Oral and Maxillofacial Surgery, Singapore

Victor Tai Weng Fan

National University of Singapore, Oral and Maxillofacial Surgery, Singapore

Jin Fei Yeo

National University of Singapore, Oral and Maxillofacial Surgery, Singapore

Tong Cao

National University of Singapore, Oral and Maxillofacial Surgery, Singapore, omscaot{at}nus.edu.sg

Eunice Tan

National University of Singapore, Division of Bioengineering, Singapore

Bone repair and regeneration can be enhanced through implantation of biocompatible and biodegradable scaffolds, which serve primarily as osteoconductive moieties. In this study, the mechanical properties and microenviroment of 3D printed poly-lactic-co-glycolic acid (PLGA) scaffolds are examined. Additionally, the proliferation and differentiation of human fetal osteoblasts are evaluated after 3 weeks of in vitro culture on the scaffolds. The results showed that the PLGA scaffolds examined had mechanical properties similar to that of trabecular bone, but was still much weaker compared to cortical bone. In addition to general porosity, the PLGA scaffolds also had micropores within macropore walls. Cultured human osteoblasts could proliferate upon seeding on the PLGA scaffolds. Alkaline phosphatase activity and osteonectin expression of the osteoblasts cultured on the PLGA scaffolds remained stable over three weeks, whilst expression of collagen type I and osteopontin decreased. The alkaline phosphatase activity of osteoblasts cultured on PLGA scaffolds is comparable with that from two commercially-available scaffolds — OPLA and collagen scaffolds (Becton—Dickinson (BD) Inc., Franklin Lakes, NJ, USA). Hence, the results suggested that the PLGA scaffolds examined are conducive for promoting osteogenesis.

Key Words: Osteoblast • scaffold • 3D printing • osteogenesis.

This version was published on May 1, 2009

Journal of Biomaterials Applications, Vol. 23, No. 6, 533-547 (2009)
DOI: 10.1177/0885328208094301
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?