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Journal of Biomaterials Applications
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Biological Properties of Crosslinked Salmon Collagen Fibrillar Gel as a Scaffold for Human Umbilical Vein Endothelial Cells

Nobuhiro Nagai

Creative Research Initiative 'Sousei' (CRIS), Hokkaido University N21-W10, Kita-ku, Sapporo, Hokkaido 001-0021, Japan, nagai491{at}yahoo.co.jp

Kazuo Mori

Ihara & Company Ltd., 3-263-23 Zenibako, Otaru Hokkaido 047-0261, Japan

Masanobu Munekata

Division of Biotechnology and Macromolecular Chemistry Graduate School of Engineering, Hokkaido University N13-W8, Kita-ku, Sapporo, Hokkaido 060-8628, Japan

Collagen derived from chum salmon (Oncorhynchus keta) was crosslinked with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) during collagen fibrillogenesis and applied to an in vitro cell culture to evaluate its potential use as a scaffold for vascular tissue engineering. Human umbilical vein endothelial cells (HUVEC) were cultured on the crosslinked salmon collagen fibrillar gel (EDC-SC gel), and their growth rates and production levels of cytokines, including platelet-derived growth factor-BB and von Willebrand factor, were measured. Comparison was also made with bovine collagen gel crosslinked with EDC (EDC-BC gel). The growth and cytokine production of the HUVEC cultured on the EDC-SC gel were higher than those on the EDC-BC gel. In addition, HUVEC were found to attach to the EDC-BC gel through {alpha}2β1 integrin for native collagen, whereas they attached to the EDC-SC gel through {alpha}vβ3 integrin for denatured collagen as well as the {alpha}2β1 integrin, indicating that HUVEC recognized denatured domains in the EDC-SC gel. In conclusion, the EDC-SC gel can be used as a scaffold to support HUVEC growth, although the integrin-mediated attachment manner differs between the two gels.

Key Words: collagen • salmon • scaffold • endothelial cells • integrin • tissue engineering.

This version was published on November 1, 2008

Journal of Biomaterials Applications, Vol. 23, No. 3, 275-287 (2008)
DOI: 10.1177/0885328208092109
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