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This version was published on July 1, 2008
Journal of Biomaterials Applications, Vol. 23, No. 1, 73-84 (2008)
DOI: 10.1177/0885328207084746

Comparative Study of P19 EC Stem Cell Differentiation in between Conventional Hanging Drop and the Zebrafish Chorion as a Bio-derived Material

Dae Seok Na

Optoelectronic Materials Research Laboratory Korea Institute of Science Technology, Seoul 136-791, Korea, Department of Electrical Engineering Korea University, Seoul 136-701, Korea

Hwang Lee

School of Life Sciences and Biotechnology Korea University, Seoul 136-701, Korea

Sun Uk Kim

School of Life Sciences and Biotechnology Korea University, Seoul 136-701, Korea

Chang Nam Hwang

School of Life Sciences and Biotechnology Korea University, Seoul 136-701, Korea

Sang Ho Lee

School of Life Sciences and Biotechnology Korea University, Seoul 136-701, Korea

Ji Yoon Kang

Microsystem Research Center, Korea Institute of Science Technology, Seoul 136-791, Korea

Jai Kyeong Kim

Optoelectronic Materials Research Laboratory Korea Institute of Science Technology, Seoul 136-791, Korea

James Jungho Pak

Department of Electrical Engineering Korea University, Seoul 136-701, Korea, pak{at}korea.ac.kr

Various materials including glass and polymers have been widely used for stem cell culture due to their biocompatibility. However, the roles of these materials are fundamentally limited because they cannot realize or imitate the complex biological functions of living tissues, except in very simple cases. Here, the development of a bio-derived material suitable for stem cell culture and improvement of differentiation efficiency to specific cell lineages with no stimulating agents by using a chorion obtained from a fertilized zebrafish egg through the removal of the yolk and embryonic cell mass from the egg is reported. Mouse P19 EC stem cells introduced into the empty chorion form a uniform embryoid body (EB) without addition of any inducing agent. It is demonstrated that the zebrafish chorion with nanopores improves efficiencies greatly in the EB formation, cell proliferation, and lineage-specific differentiations compared to those of the conventional hanging drop culture method.

Key Words: P19 EC stem cell • differentiation • comparison • hanging drop • zebrafish chorion • a bio-derived material.


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