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Microbial Leakage of Gutta-percha and Resilon^TM Root Canal Filling Material: A Comparative Study Using a new Homogeneous Assay for Sequence Detection

Department of Endodontics, School of Dentistry, University of Turin Turin, Italy; Corso A.M. Dogliotti 14 - 10126 Torino, Italy, dampasq{at}libero.it

Nicola Scotti

Department of Endodontics, School of Dentistry, University of Turin Turin, Italy; Corso A.M. Dogliotti 14 - 10126 Torino, Italy

Livio Mollo

Department of Endodontics, School of Dentistry, University of Turin Turin, Italy; Corso A.M. Dogliotti 14 - 10126 Torino, Italy

Elio Berutti

Department of Endodontics, School of Dentistry, University of Turin Turin, Italy; Corso A.M. Dogliotti 14 - 10126 Torino, Italy

Emma Angelini

Department of Material Science and Chemical Engineering Polytechnical School of Turin, Turin, Italy

Giuseppe Migliaretti

Department of Public Health and Microbiology University of Turin, Turin, Italy

Annamaria Cuffini

Department of Public Health and Microbiology University of Turin, Turin, Italy

Daniel Adlerstein

Diasorin SpA, via Crescentino snc, 13040 Saluggia (VC), Italy

The sealing ability of gutta-percha/sealer root canal filling was compared to a new thermoplastic synthetic polymer-based obturation material (Resilon ^TM), using a microleakage model and a new sequence detection assay One Cut Event AmplificatioN (OCEAN^TM). Eighty-eight extracted human teeth, shaped with K-Files and the ProTaper Technique, were randomly assigned to four groups (n = 22) and obturated in the apical 5 mm. Group R were obturated with the Resilon/Epiphany technique; group GP were obturated with gutta-percha and Zinc oxide eugenoe sealer; group RCH and GPCH received calcium hydroxide intracanal medication before being obturated. Sterilized specimens were inoculated with Enterococcus faecalis and incubated in sterile medium for 47 days. DNA extracted from the specimens was amplified by PCR and then identified by the OCEAN technique. Samples obturated with Resilon root canal filling material showed a greater number of microleakage events than the other groups (p = 0.036). Calcium hydroxide medication did not have a relevant impact on the quality of the apical seal (p = 0.044).

Key Words: root canal filling • resilon • gutta-percha • microleakage • PCR • Enterococcus faecalis • materials science.

This version was published on January 1, 2008

Journal of Biomaterials Applications, Vol. 22, No. 4, 337-352 (2008)
DOI: 10.1177/0885328207077411


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