Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Sign In to gain access to subscriptions and/or personal tools.
Journal of Biomaterials Applications
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
0885328207077590v1
22/3/275    most recent
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Schlie, S.
Right arrow Articles by Chichkov, B. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schlie, S.
Right arrow Articles by Chichkov, B. N.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Three-Dimensional Cell Growth on Structures Fabricated from ORMOCER® by Two-Photon Polymerization Technique

Sabrina Schlie

Institute of Biophysics, University Hannover, Herrenhäuserstr. 2, D-30419 Hannover, Germany

Anaclet Ngezahayo

Institute of Biophysics, University Hannover, Herrenhäuserstr. 2, D-30419 Hannover, Germany, ngezahayo{at}biophysik.uni-hannover.de

Aleksandr Ovsianikov

Laser Zentrum Hannover e.V., Hollerithallee 8, D-30419 Hannover, Germany

Tilman Fabian

Zentrum für Biomedizintechnik, Universität Hannover, Lise-Meitner-Str. 1 D-30823 Garbsen, Germany

Hans-Albert Kolb

Institute of Biophysics, University Hannover, Herrenhäuserstr. 2, D-30419 Hannover, Germany

Heinz Haferkamp

Zentrum für Biomedizintechnik, Universität Hannover, Lise-Meitner-Str. 1 D-30823 Garbsen, Germany

Boris N. Chichkov

Laser Zentrum Hannover e.V., Hollerithallee 8, D-30419 Hannover, Germany

Two-photon polymerization technique was applied to generate three-dimensional (3D) scaffold-like structures using the photosensitive organic—inorganic hybrid polymer ORMOCER®. The structures were studied with respect to potential applications as scaffold for tissue engineering. Cell counting and comet assay, respectively, demonstrated that doubling time and DNA strand breaks of CHO cells, GFSHR-17 granulosa cells, GM-7373 endothelial cells, and SH-SY5Y neuroblastoma cells were not affected by ORMOCER®. ORMOCER® related alteration of formation of tissue specific cell-to-cell adhesions like gap junctions was ruled out by double whole-cell patch-clamp technique. Additionally, growth of cells on the vertical surfaces of 3D structures composed of ORMOCER® is shown.

Key Words: tissue engineering • two-photon polymerization • ORMOCER® • 3D scaffolds • gap junction • comet assay • DNA strand breaks.

This version was published on November 1, 2007

Journal of Biomaterials Applications, Vol. 22, No. 3, 275-287 (2007)
DOI: 10.1177/0885328207077590
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?