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Journal of Biomaterials Applications
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C3A Cell Behaviors on Micropatterned Chitosan— Collagen—Gelatin Membranes

Bo-Yi Yu

Department of Chemical Engineering and Materials Science

Pei-Hsun Chou

Department of Chemical Engineering and Materials Science

Chang-An Chen

Department of Chemical Engineering and Materials Science

Yi-Ming Sun

Department of Chemical Engineering and Materials Science, Graduate School of Biotechnology and Bioinformatics Yuan Ze University, Chungli, Taoyuan, Taiwan 320, Republic of China, cesunym{at}saturn.yzu.edu.tw

Shieh-Shiuh Kung

Graduate School of Biotechnology and Bioinformatics Yuan Ze University, Chungli, Taoyuan, Taiwan 320, Republic of China

The influence of the properties and surface micropatterning of chitosan—collagen—gelatin (CCG) blended membranes on C3A cell's activities has been investigated. It is aimed to guide the cell growth and improve the growth rate in vitro for the application in tissue engineering. Masters with micropatterns are prepared on stainless steel plates by photolithography. The CCG membranes with surface micropatterns are then fabricated by soft lithography and dry—wet phase inversion techniques. The morphology and metabolic activity of cultured C3A cells on the membranes are recorded. When the C3A cells are seeded on the membranes with micropattern spacing of 200 µm width and 80 µm depth, they adhere and aggregate in the groove of the membranes in a few minutes. The aggregated cells migrate up to the surface of the ridge later. This phenomenon, however, is not found on membranes with a micropattern spacing of 500 µm width. In addition, it is demonstrated that the cells on the CCG membranes with micropatterns have higher metabolism and growth rates than those on the flat CCG membranes and on T-flask discs. Micropatterning on the membrane surface can affect the distribution of cells and the communication among cells, and results in a difference in cell adhesion, morphology, mobility, and growth activity.

Key Words: biomaterials • cell—material interactions • chitosan— collagen—gelatin and micropatterning.

This version was published on November 1, 2007

Journal of Biomaterials Applications, Vol. 22, No. 3, 255-274 (2007)
DOI: 10.1177/0885328207076780
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