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Journal of Biomaterials Applications
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A New Generation of Sterile and Radiopaque Impression Materials—an in vitro Cytotoxicity Study

Chiara Coppi

Department of Neurosciences, Section of Dentistry, University of Modena and Reggio Emilia, Policlinico, Largo del Pozzo 71, 41100 Modena, Italy, tiozzo.roberta{at}unimore.it

Chiara Paolinelli Devincenzi

Department of Biomedical Sciences, Section of General Pathology, University of Modena and Reggio Emilia, Via G. Campi 287, 41100 Modena, Italy

Sergio Bortolini

Department of Neurosciences, Section of Dentistry, University of Modena and Reggio Emilia, Policlinico, Largo del Pozzo 71, 41100 Modena, Italy

Ugo Consolo

Department of Neurosciences, Section of Dentistry, University of Modena and Reggio Emilia, Policlinico, Largo del Pozzo 71, 41100 Modena, Italy

Roberta Tiozzo

Department of Biomedical Sciences, Section of General Pathology, University of Modena and Reggio Emilia, Via G. Campi 287, 41100 Modena, Italy

Impression materials are largely used to record the geometry of dental tissue. Hence, the assessment of their possible cytotoxicity is a necessary step in the evaluation of their biocompatibility. The present study is carried out to evaluate the cytotoxicity of a new elastomeric sterile and radiopaque impression material. Human gingival fibroblasts, cultured in vitro are exposed directly to Elite Implant in three different viscosities, heavy, medium, and light. At 3, 9, 24, 48, and 72 h, the cellular proliferation is evaluated. In parallel, human gingival fibroblasts are exposed indirectly by means of fluid extracts of Elite Implant. The cellular viability is evaluated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, (MTT) assay (Sigma, St Louis, Mo). The gingival fibroblasts proliferation and viability are unaffected by the presence of Elite Implant. This new impression material may represent a safe medical device for clinical and surgical applications. In addition, this material is radiopaque and, thus, can be identified radiographically.

Key Words: dental impression material • human gingival fibroblast • in vitro cytotoxicity tests • proliferation • viability.

This version was published on July 1, 2007

Journal of Biomaterials Applications, Vol. 22, No. 1, 83-95 (2007)
DOI: 10.1177/0885328206071928
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