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Journal of Biomaterials Applications
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Picoliter Wells from Selective Growth of HEK293 Cells on Chemically Modified PDMS Surfaces

Gerardo A. Diaz-Quijada

Industrial Materials Institute, National Research Council of Canada, 75 de Mortagne Boulevard, Boucherville, QC, Canada, J4B 6Y4

Shirin Farrahi

Jennifer Clarke

Angela M. Tonary

The Steacie Institute for Molecular Sciences, National Research Council of Canada, 100 Sussex Dr., Ottawa, Canada, K1A 0R6

John Paul Pezacki

The Steacie Institute for Molecular Sciences, National Research Council of Canada, 100 Sussex Dr., Ottawa, Canada, K1A 0R6; John.Pezacki{at}nrc-cnrc.gc.ca

In this study, a method for the rapid generation of a variety of bifunctional surfaces that can serve to quickly determine the selective adhesion of HEK293 cells towards different chemical functionalities has been established. Using the information about selective adhesion of HEK293 cells to bifunctional surfaces, we demonstrate the ability to construct stable, high density, and multi-welled surfaces where the mammalian cells form the walls of picoliter volume wells.

Key Words: selective growth • patterned growth • human embryonic kidney cells cultured in vitro • adhesion • diffusional barriers • screening

This version was published on January 1, 2007

Journal of Biomaterials Applications, Vol. 21, No. 3, 235-249 (2007)
DOI: 10.1177/0885328206062139
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