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Journal of Biomaterials Applications
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Fibronectin Immobilization using Water-soluble Carbodiimide on Poly-L-lactic Acid for Enhancing Initial Fibroblast Attachment

Megumi Nagai

Department of Laboratory Medicine for Dentistry, Nihon University School of Dentistry at Matsudo 2-870-1, Sakaecho Nishi, Matsudo, Chiba 271-8587, Japan; m-nagai{at}mascat.nihon-u.ac.jp

Tohru Hayakawa

Department of Dental Biomaterials Research Institute of Oral Science Nihon University School of Dentistry at Matsudo 2-870-1, Sakaecho Nishi, Matsudo, Chiba 271-8587, Japan

Masaharu Makimura

Department of Laboratory Medicine for Dentistry, Nihon University School of Dentistry at Matsudo 2-870-1, Sakaecho Nishi, Matsudo, Chiba 271-8587, Japan

Masao Yoshinari

Department of Dental Materials Science and Oral Health Science Center Tokyo Dental College 1-2-2 Masago, Mihamaku, Chiba 261-8502, Japan

The aim of this study is to evaluate the influence of fibronectin immobilization on poly-L-lactic acid (PLA) films on the initial attachment of human gingival fibroblasts. Carboxylic acid groups are chemically introduced on the PLA films' surface by surface hydrolysis with 0.5 M NaOH. The contact angle of PLA surface with respect to double-distilled water decreases significantly after NaOH hydrolysis. X-ray photoelectron spectroscopy (XPS) also reveals significantly higher intensities of CC=O/CC-O after NaOH hydrolysis. Fibronectin is immobilized onto the hydrolyzed PLA surface through a condensation reaction between the carboxylic acid groups on the hydrolyzed PLA surface and the amino groups of fibronectin using water-soluble carbodiimide. XPS analysis shows that the fibronectin-immobilized PLA surface is enriched with nitrogen atoms. The immobilization of fibronectin significantly enhances the number of initially attached human gingival fibroblasts on the PLA surface. No obvious differences in morphology are noted between fibroblasts cultured on native PLA and on fibronectin-immobilized PLA. Fibronectin can be immobilized onto the PLA surface after NaOH hydrolysis and this is effective in enhancing the initial attachment of human gingival fibroblasts.

Key Words: polylactic acid • fibronectin • fibroblast • initial cell attachment

This version was published on July 1, 2006

Journal of Biomaterials Applications, Vol. 21, No. 1, 33-47 (2006)
DOI: 10.1177/0885328206055316
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