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In Vitro Attachment of Staphylococcus Epidermidis to Surgical Sutures with and without Ag-Containing Bioactive Glass Coating

Division of Infection and Immunity, Eastman Dental Institute for Oral Health Care Sciences University College London, 256 Gray’s Inn Road London WC1X 8LD, UK

Showan N. Nazhat

Division of Biomaterials and Tissue Engineering, Eastman Dental Institute for Oral Health Care Sciences, University College London, 256 Gray’s Inn Road, London WC1X 8LD, UK

Jonny J. Blaker

Department of Materials and Centre for Tissue Engineering and Regenerative Medicine, Imperial College London Prince Consort Road London, SW7 2BP, UK

Aldo R. Boccaccini

Department of Materials and Centre for Tissue Engineering and Regenerative Medicine, Imperial College London Prince Consort Road London, SW7 2BP, UK, a.boccaccini{at}imperial.ac.uk

The ability of a silver-doped bioactive glass (AgBG) coating to prevent bacterial colonization on surgical sutures was investigated in vitro. Bioactive glass powders, in the form of 45S5 Bioglass® and AgBG, were used to coat Mersilk® sutures using an optimized ‘in house’ slurry-dipping process. In vitro experiments were carried out using Staphylococcus epidermidis under both batch and flow conditions. While the traditional batch culture testing was used to determine the number of viable cells adhered to the surface, the flow-cell was used to visualize attachment and detachment over time. Under batch conditions of up to 180 min, statistically significant differences were observed in the colony forming units (CFU) per suture for both the coated and uncoated Mersilk® sutures. The results showed that the AgBG coating had the greatest effect on limiting bacterial attachment (8 10² CFU) when compared to the 45S5 Bioglass® coating (3.2 10³ CFU) and the uncoated Mersilk® (1.2 10⁴ CFU). Also under flow conditions differences were seen between the coated and uncoated sutures. Therefore, this preliminary study has demonstrated the quantification and visualization of bacterial attachment onto sutures in order to compare the antibacterial properties of Ag-containing bioactive glass coatings. The bactericidal properties imparted by Ag-containing glass open new opportunities for use of the composite sutures in wound healing and body wall repair.

Key Words: bacterial adhesion • flow-cell • sutures • silver • bioactive glasses

Journal of Biomaterials Applications, Vol. 19, No. 1, 47-57 (2004)
DOI: 10.1177/0885328204043200


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