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Journal of Biomaterials Applications
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A Covalent Method of Gentamicin Bonding to Silica Supports

Grazyna Ginalska

Department of Biochemistry Maria Curie-Sk&unknown;odowska University 20-031 Lublin, Poland ginal{at}hermes.umcs.lublin.pl

Monika Osinska

Department of Biochemistry Maria Curie-Sk&unknown;odowska University 20-031 Lublin, Poland

Adam Uryniak

Division of General and Vascular Surgery Municipal Hospital 35-241 Rzeszów, Poland

Results of a novel method of covalent bonding of an antibiotic (gentamicin) to silica bead supports are shown. Gentamicin was immobilized to four types of matrix: silica gel and porous glass beads activated by either silanization (APTES) or by adhesively bound keratin (with immobilization yield ranging from 36.5 to 91%). Gentamicin was immobilized to the supports after opening its carbohydrate ring in the molecule. This method of gentamicin activation before the immobilization process did not inhibit its antibiotic activity. The four gentamicin-containing immobilized preparations were stable, meaning that they did not release the antibiotic into the solution during the 30 days of incubation, not even during shaking experiments.

Key Words: gentamicin • covalent immobilization • silica gel • porous glass beads

Journal of Biomaterials Applications, Vol. 18, No. 4, 279-289 (2004)
DOI: 10.1177/0885328204041443
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