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Journal of Biomaterials Applications
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Effect of c-Sterilization Process on PLGA Microspheres Loaded with Insulin-Like Growth Factor - I (IGF-I)

C. Carrascosa

Departamento de Farmacia y Tecnología Farmacéutica Facultad de Farmacia Plaza Ramón y Cajal E-28040 Madrid, Spain

L. Espejo

Departamento de Farmacia y Tecnología Farmacéutica Facultad de Farmacia Plaza Ramón y Cajal E-28040 Madrid, Spain

S. Torrado

Departamento de Farmacia y Tecnología Farmacéutica Facultad de Farmacia Plaza Ramón y Cajal E-28040 Madrid, Spain

J. J. Torrado

Departamento de Farmacia y Tecnología Farmacéutica Facultad de Farmacia Plaza Ramón y Cajal E-28040 Madrid, Spain, torrado1{at}farm.ucm.es

The influence of {gamma}-sterilization on the physicochemical properties of a controlled release formulation for the insulin-like growth factor-I (IGF-I) was investigated in this study. Recombinant human insulin-like growth factor-I (rhIGF-I) was efficiently entrapped in poly (D,L-lactide-co-glycolide) (PLGA) microspheres by water-in-oil-in-water (W/O/W) solvent evaporation technique. Microspheres were irradiated at a dose of 25 kGy and evaluated by means of scanning electron microscopy (SEM) and differential scanning calorimetry (DSC). The stability of the released protein was investigated by circular dichroism (CD) and sodium dodecyl sulfate polyacrilamide gel electrophoresis (SDS-PAGE). No difference was noticed in microsphere size and morphology before and after irradiation. Drug loading remains essentially the same after the sterilization process. However, rhIGF-I aggregation was detected by electrophoresis. In addition, subtle changes in DSC pattern were noticed for irradiated microspheres. In vitro drug release from irradiated microspheres was also affected, showing an increased burst effect. From this results it can be concluded that {gamma}-sterilization process causes changes in the properties of rhIGF-I loaded microspheres.

Key Words: poly(D-lactide-co-glycolide) • microspheres • gamma irradiation • recombinant human insulin-like growth factor-I

Journal of Biomaterials Applications, Vol. 18, No. 2, 95-108 (2003)
DOI: 10.1177/088532803038026
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