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Journal of Biomaterials Applications
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Quality Assessment of Heparin Coatings by their Binding Capacities of Coagulation and Complement Enzymes

N. Weber

Division of Thoracic, Cardiac and Vascular Surgery, Department of Surgery, Eberhard-Karls-University, Hoppe-Seyler-Str. 3, 72076 Tuebingen, Germany

H. P. Wendel

Division of Thoracic, Cardiac and Vascular Surgery, Department of Surgery, Eberhard-Karls-University, Hoppe-Seyler-Str. 3, 72076 Tuebingen, Germany

G. Ziemer

Division of Thoracic, Cardiac and Vascular Surgery, Department of Surgery, Eberhard-Karls-Uniuersity, Hoppe-Seyler-Str. 3, 72076 Tuebingen, Germany

In vitro testing of blood contacting materials before clinical application is generally advisable. Four heparin coatings from different manufacturers were tested for adsorbed proteins and soluble activation markers. The surface with the highest antithrombin, thrombin, high-molecular-weight-kininogen (HMWK) and the lowest fibrinogen binding capacity (Carmeda, Medtronic) showed significantly lower levels of granulocytes and platelet activation (ß-TG, PMN-elastase release). No statistically significant differences in soluble markers of the coagulation system could be detected (F1+2, TAT). Interestingly, complement activation (TCC) was significantly reduced within the group of the lowest adsorption of the complement factor C3.

Our data demonstrate that there is a relation between the binding affinity of proteins (C1-inhibitor, C3-complement) and the consecutive changes in complement activation (TCC). Therefore, measuring adsorbed proteins on artificial surfaces is a suitable, sensitive and very reproducible method for assessing the thrombogenicity of biomaterials.

Key Words: hemocompatibility testing • heparin coating • extracorporeal circulation

Journal of Biomaterials Applications, Vol. 15, No. 1, 8-22 (2000)
DOI: 10.1106/DUYU-CEL6-529C-WUL9
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 J Biomater ApplHome page
H. P. Wendel, N. Hauser, F. Briquet, and G. Ziemer
Hemocompatibility of Medical Connectors with Biopassive or Bioactive Surface Coatings
J Biomater Appl, July 1, 2002; 17(1): 5 - 17.
[Abstract] [PDF]