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Journal of Biomaterials Applications
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Enhanced Surfaces for Endothelial Cell Seeding

Hiroko Sato

Graduate School of Engineering, Kyoto University, Yoshida-honmachi, Kyoto 606-8501 Japan

Hiroshi Tsuji

Graduate School of Engineering, Kyoto University, Yoshida-honmachi, Kyoto 606-8501 Japan

Shinichi Ikemura

Graduate School of Engineering, Kyoto University, Yoshida-honmachi, Kyoto 606-8501 Japan

Shigeo Ikeda

Graduate School of Engineering, Kyoto University, Yoshida-honmachi, Kyoto 606-8501 Japan

Junzo Ishikawa

Graduate School of Engineering, Kyoto University, Yoshida-honmachi, Kyoto 606-8501 Japan

Sei-Ichi Nishimoto

Graduate School of Engineering, Kyoto University, Yoshida-honmachi, Kyoto 606-8501 Japan

Silver negative ions (Ag–) were implanted to polystyrene (PS) and tissue culture polystyrene (TCPS) by using neutral and ionized alkaline bombardment-type heavy negative ion sources, NIABNIS. Surface property and biocompatibility of Ag–-implanted TCPS and PS were investigated. Enhanced growth of vascular endothelial cells was observed on Ag–-implanted TCPS at a dose of 1016 ions·cm–2 at an ion energy of 10 keV and Ag–-implanted (at ca. 1015 ions·cm–2 dose and 10 keV energy) PS region. As a result of Ag–-implantation, PS and TCPS surfaces were considered to be more hydrophobic and to be more hydrophilic, respectively.

Key Words: silver negative ions (Ag–) • implanted surfaces • PS/TCPS • cell-adhesive proteins • hydrophilicity/hydrophobicity • vascular endothelial cells

Journal of Biomaterials Applications, Vol. 14, No. 2, 169-183 (1999)
DOI: 10.1177/088532829901400203
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