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Journal of Biomaterials Applications
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In Vitro Assessment of the Biological Activity of Basic Fibroblast Growth Factor Released from Various Polymers and Biomatrices

Marilyn J. Davies

Department of Pathology, University of Western Australia, Nedlands, Western Australia 6009, Australia

Christopher A. Mitchell

Department of Pathology, University of Western Australia, Nedlands, Western Australia 6009, Australia

Moira A. L. Maley

Department of Pathology, University of Western Australia, Nedlands, Western Australia 6009, Australia

Miranda D. Grounds

Department of Pathology, University of Western Australia, Nedlands, Western Australia 6009, Australia

Alan R. Harvey

Department of Anatomy and Human Biology, University of Western Australia, Nedlands, Western Australia 6009, Australia

Giles W. Plant

Department of Anatomy and Human Biology, University of Western Australia, Nedlands, Western Australia 6009, Australia

David J. Wood

Department of Orthopaedic Surgery, University of Western Australia, Nedlands, Western Australia 6009, Australia

Ye Hong

Lions Eye Institute, 2 Verdun Street, Block A, Nedlands, Western Australia 6009, Australia

Traian V. Chirila

Lions Eye Institute, 2 Verdun Street, Block A, Nedlands, Western Australia 6009, Australia

The kinetics of controlled release of basic fibroblast growth factor (bFGF) from polymers (sutures, polycarbonate, Hydron, and Elvax), biopolymers (alginate), and biomatrices (lens capsules), and conditions for storage of bFGF (temperature, plastic type, heparin) were evaluated in vitro. Tissue culture proliferation bioassays with 3T3 fibroblasts, showed that only lens capsules with bFGF had a sustained release of bFGF for up to three weeks. The other materials released all of the `bound' bFGF with two hours or produced an inflammatory response in vivo. Therefore, the lens tissue had the most potential for controlled long-term delivery of bFGF in vivo. These studies emphasise the importance of in vitro analysis of release kinetics of growth factors from a range of materials as a basis for potential in vivo applications.

Key Words: basic fibroblast growthfactor • biomaterials • proliferation assay • release kinetics • Elvax • Hydron

Journal of Biomaterials Applications, Vol. 12, No. 1, 31-56 (1997)
DOI: 10.1177/088532829701200103
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