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In vitro Proliferation and Osteogenic Differentiation of Human Bone Marrow-derived Mesenchymal Stem Cells Cultured with Hardystonite (Ca₂ZnSi₂O₇) and -TCP Ceramics

¹ Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, Japan
² Biomaterials Center, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan
³ Biomaterials and Tissue Engineering Research Center, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, China

^* To whom correspondence should be addressed. E-mail: guoping.chen{at}nims.go.jp.

	Abstract

The effects of hardystonite (Ca₂ZnSi₂O₇, CSZn) and tricalcium phosphate (-TCP) on the proliferation and osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (MSCs) were compared by directly culturing MSCs on ceramic disks (contact mode) or separately culturing cells with ceramic disks (non-contact mode). In non-contact mode, the CSZn ceramic supported MSC proliferation more strongly than did the -TCP ceramic. However, in contact mode, the MSCs proliferated more quickly on the -TCP ceramic than they did on the CSZn ceramic. Alkaline phosphatase (ALP) staining and osteogenic gene expression analysis showed that the CSZn and -TCP ceramics had significant effects on the promotion of the osteogenic differentiation of MSCs in both non-contact and contact mode. Furthermore, in contact mode, the CSZn disk promoted the osteogenic differentiation of MSCs more strongly than did the -TCP disks. Even without the induction of dexamethasone and -glycerophosphate, CSZn stimulated the osteogenic differentiation of MSCs. These results suggest that CSZn ceramic would be a useful candidate material for bone regeneration and hard tissue engineering.

First published on September 2, 2009
Journal of Biomaterials Applications 2009, doi:10.1177/0885328209342469


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