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Novel Scaffolds of Collagen with Bioactive Nanofiller for the Osteogenic Stimulation of Bone Marrow Stromal Cells

¹ Department of Biomaterials Science, School of Dentistry and Institute of Tissue Regeneration Engineering (ITREN), Dankook University, South Korea; Biomaterials and Tissue Engineering Lab, Department of Nanobiomedical Science & WCU Research Center, Dankook University, South Korea
² Department of Biomaterials Science, School of Dentistry and Institute of Tissue Regeneration Engineering (ITREN), Dankook University, South Korea
³ Biomaterials and Tissue Engineering Lab, Department of Nanobiomedical Science & WCU Research Center, Dankook University, South Korea

^* To whom correspondence should be addressed. E-mail: kimhw{at}dku.edu.

	Abstract

The properties of scaffolds and their roles in regulating functions of tissue cells are considered to be of utmost importance in the successful recovery of damaged tissues. Herein, novel scaffolds of collagen and bioactive inorganic nanofiller were produced for bone tissue engineering. In addition, the in vitro responses of bone marrow-derived stromal cells (BMSCs) on these scaffolds were investigated. Glasses with bioactive compositions were prepared in nanofibrous form and homogenized with a collagen to produce hybridized porous scaffolds. The glass fibrous filaments with diameters of a few hundred nanometers were embedded well within the collagen network, characterizing a typical nanocomposite. The scaffolds showed the characteristics of a hydrogel with remarkable water uptake and swelling degree, which were similar to those of the pure collagen. In addition, the scaffolds induced the precipitation of bone-like minerals on the surface under a body-simulating medium, showing the sign of in vitro bone bioactivity. BMSCs adhered and spread well over the scaffold surface and migrated deep into the scaffold network. The osteogenic marker, alkaline phosphatase, was strongly expressed on the hybrid scaffolds, with the level higher than that on pure collagen. Overall, the collagen–inorganic nanofiller scaffolds are considered to find potential utility in bone tissue engineering.

First published on August 11, 2009
Journal of Biomaterials Applications 2009, doi:10.1177/0885328209338956


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